thruplex dna seq 12s kit (TaKaRa)
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thruplex dna seq 12s kit
Thruplex Dna Seq 12s Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+12s+kit/ThruPLEX+DNA-Seq+Kit/bio_rxiv__64898__2026__02__04__703893-144-6-11
Average 96 stars, based on 629 article reviews
Thruplex Dna Seq 12s Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thruplex+dna-seq+12s+kit/ThruPLEX+DNA-Seq+Kit/bio_rxiv__64898__2026__02__04__703893-144-6-11
Average 96 stars, based on 629 article reviews
thruplex dna seq 12s kit - by Bioz Stars,
2026-10
96/100 stars
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Sonication:Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIP-sequencing, the libraries were constructed using a Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation. Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIPsequencing, the libraries were constructed using a Immunoprecipitation:Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIP-sequencing, the libraries were constructed using a Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation. Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIPsequencing, the libraries were constructed using a Chromatin Immunoprecipitation:Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIP-sequencing, the libraries were constructed using a Construct:Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIP-sequencing, the libraries were constructed using a Article Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation. Article Snippet: .. Briefly, K562 chromatin was sonicated with Bioruptor (Diagenode) for 15–25 cycles (30 s on, 30 s off, high power), and immunoprecipitation was performed with Dynabeads protein G. For ChIPsequencing, the libraries were constructed using a DNA Sequencing:Article Title: Hedgehog signaling is required for the maintenance of mesenchymal nephron progenitors Article Snippet: Fragmentation of RNA followed by reverse transcription and second strand cDNA synthesis was done using NEBNext RNA First Strand Synthesis Module (E7525L) and NEBNext RNA Second Strand Synthesis Module (E6111L) following manufacturer’s instructions. .. The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using other:Article Title: SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CaseVeiwer https://www.3dhistech.com/ solutions/caseviewer/ v2.4 ZEN https://www.zeiss.com/ corporate/int/home.html v2.3; RRID:SCR_018163 R-Studio https://www.rstudio.com/ v1.4.1717 R https://www.r-project.org/ v4.0.5 DESeq2 Love et al., 2014 1.28.1; RRID:SCR_015687 STAR Dobin et al., 2013 v2.7.5b; RRID:SCR_016533 featureCounts Liao et al., 2014 v2.0.1; RRID:SCR_012919 Clusterprofiler Yu et al., 2012; (Wu, 2021) v3.16.1; RRID:SCR_016884 Limma Ritchie et al., 2015 v3.44.3; RRID:SCR_010943 GSEA Subramanian et al., 2005 v4.1.0; RRID:SCR_003199 CellRanger https://support. Article Title: Strategic targeting of Cas9 nickase expands tandem gene arrays. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal antibody Sigma-Aldrich Cat# 05-928 RRID:AB_492621 Histone H3K56ac rabbit polyclonal antibody Active Motif Cat# 39281 RRID:AB_2661786 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074 RRID:AB_2099233 Bacterial and virus strains DH5a high ChampionTM cell SMOBIO Cat# CC5202 Chemicals, peptides, and recombinant proteins 17b-Estradiol Nakarai tesque Cat# 14541-74 Nicotinamide Nakarai tesque Cat# 24317-72 Critical commercial assays KOD One PCR Master Mix (Dye-free 23PCR Master Mix) TOYOBO Cat# KMM-101 KOD SYBR qPCR Mix TOYOBO Cat# QKD-201 Chelex 100 Chelating Resin, biotechnology grade, 100–200 mesh, sodium form Bio-Rad Cat# 1432832 Quick-DNA Fungal/Bacterial Miniprep Kit ZYMO RESEARCH Cat# Article Title: Strategic targeting of Cas9 nickase expands tandem gene arrays Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal antibody Sigma-Aldrich Cat# 05-928 RRID:AB_492621 Histone H3K56ac rabbit polyclonal antibody Active Motif Cat# 39281 RRID:AB_2661786 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat# 7074 RRID:AB_2099233 Bacterial and virus strains DH5a high ChampionTM cell SMOBIO Cat# CC5202 Chemicals, peptides, and recombinant proteins 17b-Estradiol Nakarai tesque Cat# 14541-74 Nicotinamide Nakarai tesque Cat# 24317-72 Critical commercial assays KOD One PCR Master Mix (Dye-free 23PCR Master Mix) TOYOBO Cat# KMM-101 KOD SYBR qPCR Mix TOYOBO Cat# QKD-201 Chelex 100 Chelating Resin, biotechnology grade, 100–200 mesh, sodium form Bio-Rad Cat# 1432832 Quick-DNA Fungal/Bacterial Miniprep Kit ZYMO RESEARCH Cat# Sequencing:Article Title: Constitutive Yap activation in distal nephron segments disrupts epithelial identity and nephron patterning Article Snippet: Fragmentation of mRNA and cDNA synthesis were performed using NEBNext RNA First Strand Synthesis Module (E7525L) and NEBNext RNA Second Strand Synthesis Module (E6111L). .. The cDNA’s were processed to sequencing libraries using Article Title: A knock-in Six2Cre line reveals transient interstitial potential in nephron progenitors Article Snippet: NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) was used to purify mRNAs from total RNA. cDNA synthesis was performed using NEBNext RNA First (E7525L) and Second Strand (E6111L) Synthesis Modules (New England Biolabs). .. Sequencing libraries were prepared using the |